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. 1996;41(1):51-8.

Differentiation of Borrelia burgdorferi sensu lato strains isolated from skin biopsies and tick by pulsed-field gel electrophoresis

U Busch  1 , C Hizo Teufel, R Boehmer, V Preacmursic
Affiliations
  • PMID: 8673805

Differentiation of Borrelia burgdorferi sensu lato strains isolated from skin biopsies and tick by pulsed-field gel electrophoresis

U Busch et al. Rocz Akad Med Bialymst. 1996.

Abstract

Fifteen isolates of Borrelia burgdorferi sensu lato from skin biopsies (8) and ticks (7) were investigated for species differentiation by pulsed-field gel electrophoresis (PFGE). The skin isolates were further characterized by plasmid profile as well as SDS-PAGE and revealed six Borrelia afzelii-, one Borrelia burgdorferi sensu stricto-, and one Borrelia garinii species. The tick isolates showed three Borrelia afzelii--and four Borrelia garinii species. The skin isolates could be further discriminated by plasmid profile analysis--all strains showed an individual pattern. The SDS-PAGE of whole-cell lysates obtained from skin isolate showed a different protein pattern for each of the skin isolates.

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