Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1996 Mar;49(3):258-9.
doi: 10.1136/jcp.49.3.258.

OCT embedded sections of pathological specimens as a source of high quality RNA for reverse transcriptase/polymerase chain reaction

J A Irving  1 , G Cain, A Parr, M Howard, B Angus, A R Cattan
Affiliations

OCT embedded sections of pathological specimens as a source of high quality RNA for reverse transcriptase/polymerase chain reaction

J A Irving et al. J Clin Pathol. 1996 Mar.

Abstract

OCT embedded cryostat sections of stored pathological specimens of non-Hodgkin's lymphoma were used to provide RNA. After reverse transcription to produce cDNA, the polymerase chain reaction was performed with primers for standard and variant forms of the CD44 molecule. Using Southern transfer and hybridisation with a probe specific for exon 4 of the CD44 gene, both standard and variant forms were visualised by autoradiography. This method was shown to be applicable to other gene products by using primers specific for the abl and bcr genes. This technique permits retrospective analysis of RNA from small amounts of stored pathological samples.

PubMed Disclaimer

References

    1. Biochemistry. 1979 Nov 27;18(24):5294-9 - PubMed
    1. Lancet. 1992 Oct 31;340(8827):1053-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):12160-4 - PubMed
    1. Leuk Lymphoma. 1993;11 Suppl 1:39-43 - PubMed
    1. Bioessays. 1995 Jan;17(1):17-24 - PubMed

Publication types