Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1996 Jun 12;251(3):261-6.
doi: 10.1007/BF02172516.

Complementation of an Arabidopsis thaliana biotin auxotroph with an Escherichia coli biotin biosynthetic gene

D A Patton  1 , S Volrath, E R Ward
Affiliations

Complementation of an Arabidopsis thaliana biotin auxotroph with an Escherichia coli biotin biosynthetic gene

D A Patton et al. Mol Gen Genet. .

Abstract

The Arabidopsis thaliana biotin auxotroph biol was rendered prototrophic by transformation with a chimeric transgene containing the Escherichia coli bio A gene driven by a constitutive promoter. The bio A gene encodes the biotin biosynthetic enzyme 7, 8-diaminopelargonic acid aminotransferase. Unlike the untransformed control plants, transgenic plants expressing the bacterial transgene synthesized biotin and grew to maturity without biotin-deficiency symptoms. These findings demonstrate that bio1/bio1 mutant plants are defective in the gene encoding 7,8-diaminopelargonic acid aminotransferase.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Annu Rev Biochem. 1977;46:385-413 - PubMed
    1. Plant Physiol. 1990 Jul;93(3):1162-7 - PubMed
    1. Science. 1987 Jun 5;236(4806):1299-302 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Nov;84(21):7542-6 - PubMed
    1. Theor Appl Genet. 1985 Mar;69(5-6):543-52 - PubMed