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. 1996 Jun 7;1307(2):132-6.
doi: 10.1016/0167-4781(96)00047-4.

Molecular cloning, expression and function of the murine CB2 peripheral cannabinoid receptor

D Shire  1 , B Calandra, M Rinaldi-Carmona, D Oustric, B Pessègue, O Bonnin-Cabanne, G Le Fur, D Caput, P Ferrara
Affiliations

Molecular cloning, expression and function of the murine CB2 peripheral cannabinoid receptor

D Shire et al. Biochim Biophys Acta. .

Abstract

We have cloned the peripheral cannabinoid receptor, mCB2, from a mouse splenocyte cDNA library. The 3.7 kb sequence contains an open reading frame encoding a protein of 347 residues sharing 82% overall identity with the only other known peripheral receptor, human CB2 (hCB2) and shorter than hCB2 by 13 amino acids at the carboxyl terminus. Binding experiments with membranes from COS-3 cells transiently expressing mCB2 showed that the synthetic cannabinoid WIN 55212-2 had a 6-fold lower affinity for mCB2 than for hCB2, whereas both receptors showed similar affinities for the agonists CP 55,940, delta(9)-THC and anandamide and almost no affinity for the central receptor- (CB1) specific antagonist SR 141716A. Both hCB2 and mCB2 mediate agonist-stimulated inhibition of forskolin-induced cAMP production in CHO cell lines permanently expressing the receptors. SR 141716A failed to antagonize this activity in either cell line, confirming its specificity for CB1.

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