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Review
. 1996 Jun 24;389(1):88-91.
doi: 10.1016/0014-5793(96)00525-x.

The Sulfolobus solfataricus P2 genome project

R L Charlebois  1 , T Gaasterland, M A Ragan, W F Doolittle, C W Sensen
Affiliations
Free article
Review

The Sulfolobus solfataricus P2 genome project

R L Charlebois et al. FEBS Lett. .
Free article

Abstract

Over 800 kbp of the 3-Mbp genome of Sulfolobus solfataricus have been sequenced to date. Our approach is to sequence subclones of mapped cosmids, followed by sequencing directly on cosmid templates with custom primers. Using a prototype automated system for genome-scale analysis, known as MAGPIE, along with other tools, we have discovered one open reading frame of at least 100 amino acids per kbp of sequence, and have been able to associate 50% of these with known genes through database searches. An examination of completely sequenced cosmids suggests a clustering of genes by function in the S. solfataricus genome.

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