Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1996 Jan;56(1):119-21.
doi: 10.1016/0166-0934(95)01956-1.

Consensus oligonucleotide primers for the detection of GB virus C in human cryptogenic hepatitis

T P Leary  1 , A S Muerhoff, J N Simons, T J Pilot-Matias, J C Erker, M L Chalmers, G G Schlauder, G J Dawson, S M Desai, I K Mushahwar
Affiliations

Consensus oligonucleotide primers for the detection of GB virus C in human cryptogenic hepatitis

T P Leary et al. J Virol Methods. 1996 Jan.

Abstract

Recently, sequences from a putative member of the Flaviviridae, GB virus C (GBV-C), were isolated from the serum of patients with cryptogenic hepatitis. These sequences were 83-99% identical at the nucleotide level. Because of the divergence between these GBV-C isolates, it is likely that the PCR-based detection assay yields false negatives, underestimating dramatically the true prevalence of GBV-C in human hepatitis. We report the design of a GBV-C consensus oligonucleotide primer pair that is superior to those originally described. These primers identify GBV-C sequences in cases of cryptogenic hepatitis, allowing a better estimation of the prevalence of this virus in human populations.

PubMed Disclaimer

LinkOut - more resources