Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1996 Jul 9;93(14):7063-8.
doi: 10.1073/pnas.93.14.7063.

Bcl-2 interacting protein, BAG-1, binds to and activates the kinase Raf-1

H G Wang  1 , S Takayama, U R Rapp, J C Reed
Affiliations

Bcl-2 interacting protein, BAG-1, binds to and activates the kinase Raf-1

H G Wang et al. Proc Natl Acad Sci U S A. .

Abstract

The Bcl-2 protein blocks programmed cell death (apoptosis) through an unknown mechanism. Previously we identified a Bcl-2 interacting protein BAG-1 that enhances the anti-apoptotic effects of Bcl-2. Like BAG-1, the serine/threonine protein kinase Raf-1 also can functionally cooperate with Bcl-2 in suppressing apoptosis. Here we show that Raf-1 and BAG-1 specifically interact in vitro and in yeast two-hybrid assays. Raf-1 and BAG-1 can also be coimmunoprecipitated from mammalian cells and from insect cells infected with recombinant baculoviruses encoding these proteins. Furthermore, bacterially-produced BAG-1 protein can increase the kinase activity of Raf-1 in vitro. BAG-1 also activates this mammalian kinase in yeast. These observations suggest that the Bcl-2 binding protein BAG-1 joins Ras and 14-3-3 proteins as potential activators of the kinase Raf-1.

PubMed Disclaimer

References

    1. J Exp Med. 1993 Aug 1;178(2):427-38 - PubMed
    1. Nature. 1994 Oct 13;371(6498):612-4 - PubMed
    1. Mol Cell Biol. 1993 Nov;13(11):7170-9 - PubMed
    1. J Biol Chem. 1994 Jan 14;269(2):1249-56 - PubMed
    1. J Cell Biol. 1994 Jan;124(1-2):1-6 - PubMed

Publication types

MeSH terms

Substances