Bioassay for homogeneous parasporal crystal of Bacillus thuringiensis using the tobacco hornworm, Manduca sexta
- PMID: 869534
- PMCID: PMC170784
- DOI: 10.1128/aem.33.4.878-880.1977
Bioassay for homogeneous parasporal crystal of Bacillus thuringiensis using the tobacco hornworm, Manduca sexta
Abstract
A method for determining the toxicity of Bacillus thuringiensis subsp. kurstaki parasporal crystal to the tabocco hornworm, Manduca sexta, is described. The use of both mortality and weight loss data have provided a highly sensitive and reproducible bioassay that can be used to compare relative toxicities of crystals from other subspecies as well as toxic components contained therein.
Similar articles
-
Toxicity of parasporal crystals of Bacillus thuringiensis to the Indian meal moth, Plodia interpunctella.Appl Environ Microbiol. 1979 May;37(5):1012-5. doi: 10.1128/aem.37.5.1012-1015.1979. Appl Environ Microbiol. 1979. PMID: 485134 Free PMC article.
-
Toxicity of Bacillus thuringiensis spores to the tobacco hornworm, Manduca sexta.Appl Environ Microbiol. 1978 Jan;35(1):121-3. doi: 10.1128/aem.35.1.121-123.1978. Appl Environ Microbiol. 1978. PMID: 623457 Free PMC article.
-
Characterized full-length and truncated plasmid clones of the crystal protein of Bacillus thuringiensis subsp. kurstaki HD-73 and their toxicity to Manduca sexta.Gene. 1985;36(3):289-300. doi: 10.1016/0378-1119(85)90184-2. Gene. 1985. PMID: 3000881
-
Toxicity of parasporal crystals of Bacillus thuringiensis subsp. israelensis to mosquitoes.Appl Environ Microbiol. 1979 Oct;38(4):656-8. doi: 10.1128/aem.38.4.656-658.1979. Appl Environ Microbiol. 1979. PMID: 44177 Free PMC article.
-
The molecular biology of parasporal crystal body formation in Bacillus thuringiensis.Annu Rev Microbiol. 1986;40:549-76. doi: 10.1146/annurev.mi.40.100186.003001. Annu Rev Microbiol. 1986. PMID: 3535652 Review.
Cited by
-
Toxicity of parasporal crystals of Bacillus thuringiensis to the Indian meal moth, Plodia interpunctella.Appl Environ Microbiol. 1979 May;37(5):1012-5. doi: 10.1128/aem.37.5.1012-1015.1979. Appl Environ Microbiol. 1979. PMID: 485134 Free PMC article.
-
Inverted repeat sequences flank a Bacillus thuringiensis crystal protein gene.J Bacteriol. 1984 Oct;160(1):95-102. doi: 10.1128/jb.160.1.95-102.1984. J Bacteriol. 1984. PMID: 6090438 Free PMC article.
-
Rearing and injection of Manduca sexta larvae to assess bacterial virulence.J Vis Exp. 2012 Dec 11;(70):e4295. doi: 10.3791/4295. J Vis Exp. 2012. PMID: 23271332 Free PMC article.
-
Immunological relationships among proteins making up the Bacillus thuringiensis subsp. israelensis crystalline toxin.Appl Environ Microbiol. 1986 Oct;52(4):644-9. doi: 10.1128/aem.52.4.644-649.1986. Appl Environ Microbiol. 1986. PMID: 3535673 Free PMC article.
-
Mapping and characterization of the entomocidal domain of the Bacillus thuringiensis CryIA(b) protoxin.Mol Gen Genet. 1995 May 20;247(4):482-7. doi: 10.1007/BF00293150. Mol Gen Genet. 1995. PMID: 7770056
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources