[Cloning and sequencing of the gene PE40, coding for a portion of Pseudomonas exotoxin A]
[Article in
Chinese]
Affiliations
- PMID: 8706148
Item in Clipboard
[Cloning and sequencing of the gene PE40, coding for a portion of Pseudomonas exotoxin A]
[Article in
Chinese]
Zhongguo Yi Xue Ke Xue Yuan Xue Bao.
1995 Jun.
Abstract
Gene PE40 coding for a mutant form of Pseudomonas Exotoxin A was devoid of its native cell binding domain, and was amplified from the total DNA of P. aeruginosa PA103 by PCR. After being sequenced, it was cloned into expressional vector pBV220 to get a recombinant plasmid, which will be useful in the research of molecular targeted protein.
Similar articles
-
Pseudomonas aeruginosa exotoxin A.Targeted Diagn Ther. 1992;7:405-23. Targeted Diagn Ther. 1992. PMID: 1633302 Review. No abstract available.
-
Cloning and expression of a gene segment encoding the enzymatic moiety of Pseudomonas aeruginosa exotoxin A.J Bacteriol. 1984 Aug;159(2):683-7. doi: 10.1128/jb.159.2.683-687.1984. J Bacteriol. 1984. PMID: 6086583 Free PMC article.
-
Identification of regB, a gene required for optimal exotoxin A yields in Pseudomonas aeruginosa.Mol Microbiol. 1990 Mar;4(3):489-97. doi: 10.1111/j.1365-2958.1990.tb00615.x. Mol Microbiol. 1990. PMID: 2113155
-
The expression, affinity purification and characterization of recombinant Pseudomonas exotoxin 40 (PE40) secreted from Escherichia coli.J Biotechnol. 1995 Aug 15;42(1):9-22. doi: 10.1016/0168-1656(95)00055-u. J Biotechnol. 1995. PMID: 7662342
-
The structure of Pseudomonas exotoxin A as a guide to rational design.Targeted Diagn Ther. 1992;7:439-46. Targeted Diagn Ther. 1992. PMID: 1633305 Review. No abstract available.