doi: 10.1016/0923-2494(96)81041-8.
Similar binding properties for a neutralizing anti-tetanus toxoid human monoclonal antibody and its bacterially expressed Fab
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- PMID: 8719661
- DOI: 10.1016/0923-2494(96)81041-8
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Similar binding properties for a neutralizing anti-tetanus toxoid human monoclonal antibody and its bacterially expressed Fab
Res Immunol.
1995 Jul-Aug.
Erratum in
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Similar binding properties for a neutralizing anti-tetanus toxoid human monoclonal antibody and its bacterially expressed Fab.Res Immunol. 1996 Jan;147(1):61. doi: 10.1016/0923-2494(96)81549-5. Res Immunol. 1996. PMID: 8739329 No abstract available.
Abstract
A high-affinity anti-tenanus toxoid (TT) human monoclonal antibody showing neutralizing activity was isolated from a fusion between mouse myeloma and human splenic cells. Fab fragments from this antibody were obtained using a recombinant phage surface-display expression system. The parental antibody and the corresponding Fab had identical immunological activities, including specificity and affinity. These results confirm the feasibility of developing Escherichia coli expression of monoclonal human Fab from hybridoma cells.
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