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. 1996;6(3):179-84.
doi: 10.3109/10425179609010207.

Cloning and sequencing of the dnaK locus in Streptomyces coelicolor A3(2)

A Brans  1 , A Loriaux, B Joris, J Dusart
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Cloning and sequencing of the dnaK locus in Streptomyces coelicolor A3(2)

A Brans et al. DNA Seq. 1996.

Abstract

The dnaK operon of Streptomyces coelicolor A3(2) was cloned by the DNA-probing method using synthetic oligonucleotides designed on the basis of two of the most conserved regions in 30 different DnaK proteins (HSP70). The isolated insert-a BamHI 5.6-kb fragment-was sequenced and shown to contain three open-reading frames organized in an operon and coding for proteins analogous to DnaK, GrpE and DnaJ, successively.

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