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. 1996 May;143(1):531-6.
doi: 10.1093/genetics/143.1.531.

Molecular genetics of rust resistance in poplars (Melampsora larici-populina Kleb/Populus sp.) by bulked segregant analysis in a 2 x 2 factorial mating design

M Villar  1 , F Lefèvre, H D Bradshaw Jr, E Teissier du Cros
Affiliations

Molecular genetics of rust resistance in poplars (Melampsora larici-populina Kleb/Populus sp.) by bulked segregant analysis in a 2 x 2 factorial mating design

M Villar et al. Genetics. 1996 May.

Abstract

With random amplified polymorphic DNA (RAPD) markers, we have tagged a genomic region in Populus sp. involved in qualitative resistance to Melampsora larici-populina. Our approach was based on three steps: use of RAPD markers that can be quickly and efficiently researched: application of "bulked segregant analysis" technique on individuals of one interspecific family P. trichocarpa x P. deltoides to search for RAPD markers linked to resistance; and validation of these markers in two other families linked with the first one in a 2 x 2 factorial mating design. Of five detected markers, only one marker M03/04_480 was polymorphic in the three segregating families, involving 89 individuals and four different parents. We have estimated the recombination value of 1 cM with 1 cM sampling error.

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References

    1. Nucleic Acids Res. 1990 Nov 25;18(22):6531-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):2066-70 - PubMed

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