[Fingerprinting of potato species and cultivar using RAPD-PCR]
- PMID: 8723639
[Fingerprinting of potato species and cultivar using RAPD-PCR]
Abstract
Polymerase chain reaction with random primers (RAPD-PCR) is used to fingerprint DNA polymorphism of six species and eight cultivars of potato. Three primers were developed experimentally for detection by DNA fingerprinting of cultivars and species. High significance and reproducibility of amplified DNA spectra for each species and cultivar was demonstrated. In these spectra, RAPD-PCR fragments, were revealed which may serve as cultivar-and species-specific markers for identification and differentiation of Solanum cultivars and species.