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Comparative Study
. 1979 Apr;55(2):105-8.
doi: 10.1136/sti.55.2.105.

Double-staining procedure for the fluorescent treponemal antibody absorption (FTA-ABS) test

E F Hunter, R M McKinney, S E Maddison, D D Cruce
Comparative Study

Double-staining procedure for the fluorescent treponemal antibody absorption (FTA-ABS) test

E F Hunter et al. Br J Vener Dis. 1979 Apr.

Abstract

The fluorescent treponemal antibody absorption (FTA-ABS) double-staining procedure was reproducible, comparable to the conventional test, and easy to read. We recommend the use of the FTA-ABS double-staining procedure for microscopes with incident illumination, the 100 x/1.30 oil achromatic objective and the 6.3 x ocular to obtain optimal fluorescence, and the KP560 as a barrier filter to exclude rhodamine emission when fluorescein fluorescence is read. With this system, errors related to poor focusing or failure to visualise treponemes on all smears should be eliminated.

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