Cryopreservation of stallion spermatozoa

Abstract

The main advantage to using frozen semen in any breeding program is faster genetic gain for the inherited trait desired. Milk production of dairy cows doubled (from 26,000 to 52,000 kg of milk/cow per year) between 1950 and 1980, because the dairy industry was using semen only from bulls with the greatest genetic potential for milk production. This genetic gain could have been achieved without the use of frozen semen; however, the time required to achieve that same genetic progress would have been lengthened exceedingly. Fertility rates using frozen stallion spermatozoa are not equal to that of fresh semen, but with careful management of both the stallion and the mare fertility rates of 40% to 60% can be achieved for a single mare reproductive cycle and fertility rates of 60% to 75% during the course of a breeding season can be expected for a number of stallions. Because pregnancy rates have increased and because many breed registries now condone the use of frozen stallion semen, more people in the equine industry are using frozen semen. It has been the attempt of this article to make the practitioner aware of some of the problems that can occur when processing, freezing, storing, and using frozen semen, and how to control those problems. The practitioner also should be aware that there is large variation in semen quality from stallions, and that spermatozoa from some of the problem stallions can be improved by altering a "standard protocol," but that semen from some stallions will not cryopreserve effectively, regardless of the efforts put forth.