Endocrine and metabolic responses to realimentation in feed-restricted prepubertal gilts: associations among gonadotropins, metabolic hormones, glucose, and uteroovarian development

Abstract

Temporal relationships among plasma LH, FSH, metabolic hormones, and glucose concentrations were studied in nutritionally manipulated prepubertal gilts. Twenty-four growth-matched littermate gilts (73.6 +/- 1.2 kg) were fed at maintenance for 7 d (d 1 to 7). From d 8 to 14, littermates were divided between two treatments and were either fed twice daily to appetite or continued on feed restriction. Feeding to appetite increased episodic LH secretion during the first 6 h of sampling on d 8 (P < .05). Plasma FSH concentrations were not affected by treatment between d 8 and 14. Nocturnal increases in plasma LH (P < .01) and FSH (P < .002) concentrations were detected in feed-restricted gilts but not realimented gilts. Mean total plasma IGF-I concentrations gradually increased in gilts fed to appetite (d 12, P < .02; d 13 to 14, P < .001). Mean postprandial insulin concentrations were also greater in gilts fed to appetite (P < .01). Treatment differences in mean postprandial glucose concentrations on d 9 and 10 indicate that normoglycemia was established by d 9 in gilts fed to appetite. At slaughter on d 15, numbers of ovarian follicles (P < .01), follicular volume (P < .001), and uterine weight (P < .05) were greater in gilts fed to appetite. In conclusion, uteroovarian responses to realimentation after short-term feed restriction in prepubertal gilts are mediated by enhanced LH secretion. This rapid enhancement of LH secretion in response to dietary repletion might have been mediated by changes in glucose and insulin status. Ovarian responses to gonadotropins may also be potentiated by observed increases in plasma glucose, insulin, and IGF-I. During feed restriction, a distinct diurnal rhythm in gonadotropin secretion was evident.