doi: 10.1038/263294a0.
Formation of stable crystalline enzyme-substrate intermediates at sub-zero temperatures
- PMID: 8732
- DOI: 10.1038/263294a0
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Formation of stable crystalline enzyme-substrate intermediates at sub-zero temperatures
Nature.
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Abstract
Sub-zero temperatures can be used to trap intermediates in enzyme-catalysed reactions using suitable cryosolvents. The feasibility of obtaining such intermediates in the crystalline state for X-ray diffraction studies has been demonstrated with several proteases, using specific substrates and optimal pH.
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