Induction and characterization of isogeneic anti-idiotypic antibodies to BALB/c myeloma S117: lack of reactivity with major idiotypic determinants

Abstract

Isogeneic anti-idiotypic antibodies were induced by immunization of BALB/c mice with the BALB/c-derived myeloma protein S117 which binds N-acetylglucosamine-containing antigens including Group A streptococcal carbohydrate (A-CHO). Most BALB/c mice produced anti-S 117 idiotypic antibodies, as shown by various different immunization protocols. The antibodies of individual mice were of intermediate to high affinity (2.8 x 10(6) M-1 to 1.4 x 10(8) M-1). In isoelectric focusing, most individual antibodies were shown to consist of a small number of clonotypes, but each mouse produced its own unique set of clones so that the potential clonal repertoire of strain BALB/c is rather large. Most importantly, all isogeneic anti-idiotypic antibodies were directed against idiotypic determinants of S117 that are not shared with induced anti-A-CHO antibodies, whereas it has been shown previously that allogeneic and xenogeneic anti-S 117 idiotypic antibodies react with idiotypic determinants unique to S 117 as well as with those that are shared with anti-A-CHO antibodies. The data suggest that the expression of S 117 idiotypic determinants in strain BALB/c is not under antibody-mediated, anti-idiotypic feedback control.