A comparison of the mineral content of enamel and dentine in human premolars and enamel pearls measured by X-ray microtomography

Abstract

Mineral content gradients in two composite enamel pearls from permanent human upper molars were measured by X-ray microtomography (XMT) at a resolution of 15-30 microns. This non-destructive microscopic technique was used to make 15-microns thick XMT slices with 100-microns separation through one pearl and 250-microns separation through the other. Average mineral contents were calculated from the linear absorption coefficients determined from regions of the XMT slices assuming the inorganic component to be calcium hydroxyapatite. These values were compared with similar XMT studies of coronal enamel and dentine of upper permanent premolars. A mineral content gradient in the pearls, reducing from the enamel surface to the amelodentinal junction, was found; this was similar to that observed in the coronal enamel of the upper premolar. The mineral contents in the surface and deeper enamel regions of the pearl were similar to those observed in premolar enamel. In contrast, the mineral content for the dentine of the pearl was greatest at the amelodentinal junction, i.e. the gradient was in the opposite direction to that observed in premolar dentine. These results suggest that the process of mineralization of the pearl dentine differs from that in permanent control dentine. In addition, gradients in enamel and dentine mineral contents reducing from the tip of the pearl to the base of the pearl were found.