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. 1996 Apr;17(4):633-41.
doi: 10.1002/elps.1150170403.

Theoretical permutation gel electrophoretic analysis of a curved DNA fragment located in circular permutation

T M Nair  1 , K Madhusudan, V Nagaraja, B D Kulkarni, H K Majumdar, R Singh
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Theoretical permutation gel electrophoretic analysis of a curved DNA fragment located in circular permutation

T M Nair et al. Electrophoresis. 1996 Apr.

Abstract

Using the theoretical model for DNA curvature, we analyzed a set of fragments with a curved insert located in circular permutation. The theoretical permutation analysis of each of the cyclically located fragments reveals the presence of a shifting molecular bend locus. The delineation of the molecular bend locus associated with the fragments obtained by a second permutation helps in providing an explanation for the differential mobility behavior of the fragments.

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