The effect of succinylcholine on energy metabolism studied by 31P-NMR spectroscopy in rat denervated skeletal muscle

Abstract

Background: The goals of this study were: (1) to demonstrate the differences of metabolic changes induced by succinylcholine (SCh) administration between normal and denervated muscle by 31P-NMR spectroscopy: (2) to determine whether three kinds of drugs (vecuronium, midazolam and magnesium sulfate) could prevent these metabolic changes.

Methods: Following unilateral sciatic nerve section, 20 male Wistar rats were studied at three-week intervals. After SCh 1 mg.kg-1 was administered intravenously, the changes of the inorganic phosphate/phosphocreatine (Pi/PCr) ratio, the beta ATP/(PCr+Pi) ratio, and intracellular pH were measured by 31P-NMR both in normal and denervated hind limb muscles of 5 rats. The other 15 rats were allocated to the pretreatment groups by the following drugs: vecuronium 0.02 mg.kg-1, midazolam 0.1 mg.kg-1 and magnesium sulfate 60 mg.kg-1. After pretreatment 3 min before SCh administration, we measured the same parameters by 31P-NMR.

Results: SCh administration did not change the Pi/PCr ratio in normal muscle, but significantly increased that in denervated muscle (P < 0.05). This increase of the Pi/PCr ratio was also observed in all pretreated groups but was minimal as compared with that in non-pretreatment denervated muscle.

Conclusion: These data suggested that SCh administration decreased the level of "energy reserve" in denervated muscle, and that this metabolic change was not totally inhibited by vecuronium, midazolam, or magnesium sulfate.