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. 1977 Jul;131(1):42-8.
doi: 10.1128/jb.131.1.42-48.1977.

Phthalate metabolism in Pseudomonas testosteroni: accumulation of 4,5-dihydroxyphthalate by a mutant strain

T Nakazawa, E Hayashi

Phthalate metabolism in Pseudomonas testosteroni: accumulation of 4,5-dihydroxyphthalate by a mutant strain

T Nakazawa et al. J Bacteriol. 1977 Jul.

Abstract

A mutant strain of Pseudomonas testosteroni blocked in phthalate catabolism converted phthalate into 4,5-dihydroxyphthalate. The latter compound was isolated, and its physical properties were determined. A stoichiometric conversion of the compound to protocatechuate was demonstrated spectrophotometrically with crude extracts of a protocatechuate 4,5-dioxygenase-deficient mutant. Therefore, phthalate is metabolized through 4,5-dihydroxyphthalate and protocatechuate, which is further degraded by protocatechuate 4,5-dioxygenase in P. testosteroni. By using several mutants blocked in phthalate catabolism, 4,5-dihydroxyphthalate decarboxylase was shown to be induced by phthalate. A simple spectrophotometric assay for the enzyme is also reported.

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References

    1. Biochem J. 1960 Aug;76(2):310-8 - PubMed
    1. Nature. 1960 Nov 12;188:560-6 - PubMed
    1. Biochim Biophys Acta. 1970 Nov 11;220(2):224-38 - PubMed
    1. J Bacteriol. 1971 Oct;108(1):89-94 - PubMed
    1. J Bacteriol. 1973 Jan;113(1):521-3 - PubMed

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