Nucleosome assembly: the CAF and the HAT

Abstract

Recent data argue strongly that a protein complex termed chromatin assembly factor-1 (CAF-I) plays a major role in de novo nucleosome assembly during DNA replication. Human CAF-I deposits newly synthesized, acetylated histones onto replicated DNA in vitro and localizes to sites of DNA replication in S-phase cells. Specific lysines of the histones used for nucleosome assembly are acetylated; in the past year the first gene encoding a histone acetyltransferase was cloned. However, mechanistic links between histone acetylation and nucleosome assembly have not been established in vivo or in vitro.