Changes in the gonadotropin-releasing hormone pulse generator activity during the estrous cycle in the goat

Abstract

The hypothalamic GnRH pulse generator activity, which was assessed by the characteristic increases in the multiple unit activity (MUA) specifically associated with the pulsatile LH secretion (MUA volleys), was monitored throughout the estrous cycle of the female goat. Blood samples were collected daily for analysis of progesterone, estradiol and LH levels to monitor ovarian cyclicity. The frequency of the MUA volleys showed a reciprocal relationship with the plasma progesterone profile; being low during the luteal phase, whereas increased during the follicular phase until the onset of the subsequent luteal phase. On the other hand, the duration of the MUA volleys changed in parallel with plasma progesterone levels showing considerable prolongation in the luteal phase. More frequent blood sampling was carried out at 2-hour intervals for timing the LH surge during the follicular phase induced by prostaglandin F2alpha (PGF2alpha) administration. During the PGF2alpha-induced follicular phase, the MUA volley frequency was abruptly increased from 4 h after PGF2alpha injection, and this high frequency was maintained throughout the follicular phase except for the period around the preovulatory LH surge when the MUA volley frequency was lowered temporarily. These results were in accordance with our previous observation that the recurrence of MUA volleys continued with decreased frequency during the estradiol-induced LH surge in ovariectomized goats. The present study revealed that GnRH pulse generator activity changes dramatically during the estrous cycle in the goat, and it is suggested that GnRH pulse generator activity becomes fully potentiated following luteolysis to induce the final follicular development and to stimulate estradiol secretion, which eventually provokes another neural mechanism responsible for the induction of the LH surge.