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. 1996;73(4):322-4.
doi: 10.1159/000134367.

Fluorescent in situ hybridization on archival G-banded slides

W el Rifai  1 , S Knuutila
Affiliations

Fluorescent in situ hybridization on archival G-banded slides

W el Rifai et al. Cytogenet Cell Genet. 1996.

Abstract

We report a simple procedure that allows the application of chromosomal in situ suppression hybridization to standard G-banded and Entellan-mounted archival preparations that had been stored for 4-8 y. The procedure includes removing the cover slips in xylene, refixation in buffered formaldehyde-acetone mixture, and washing in 2 x SSC, Tween 20.

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