[The unstable system sn(49) in Drosophila melanogaster: results of blot-hybridization and polymerase chain reaction]

Abstract

A set of 40 strains of alleles of different phenotype and mutation frequency, derived from unstable system sn49 of Drosophila melanogaster, was obtained. A mutation in the singed gene or the formation of a visible de novo mutation in the studied X chromosome served as the criterion for strain isolation. Total DNA was isolated from the obtained strains and used for blot-hybridization and polymerase chain reaction (PCR). Rearrangements were found within the first intron of the singed gene. In the first intron of an allelic derivative of this unstable system, an inserted sequence of at least 4 kb in length was found. Using PCR primers from this intron, fragment length polymorphism in different derivatives was revealed.