Overview of the regulation of metamorphosis-associated genes in Trichoplusia ni

Abstract

A review is presented of our ongoing research program on the regulation of metamorphosis-associated proteins in Trichoplusia ni. Toward the identification of mechanisms by which juvenile hormone (JH) regulates expression of metamorphosis-associated proteins, we have identified a protein that is induced by JH (juvenile hormone esterase) and a related esterase that is not JH-inducible. We have also identified three hexamerins that are suppressible by JH, and one hexamerin that is not JH-suppressible. Expression of the hexamerins is regulated at the transcriptional, translational, and posttranslational levels in T. ni. The rate of transcription of the JH esterase gene increases at the time of the prepupal peak in JH, and exogenous application of JH can cause, within 3 h, the rate of transcription to be the markedly elevated above normal. Using in vitro functional transcription assay, cell line transfection functional assay, and preliminary DNase I hypersensitive site mapping we were able to identify the functional TATA box and transcription start sites of JH-sensitive genes. These methods were also observed to be powerful in the detection of regulatory DNA motifs involved in the modulation of transcriptional activity constitutively imparted by a core promoter. The experimental systems described here will also be effective in identifying those components through which JH regulatory effects are mediated. Should JH act on the genes in a primary manner, then the transcription factor mediating that action may be the (a) JH receptor. Should the JH action be in a secondary manner, then the transcription factor(s) whose activity at the JHE gene is regulated by JH will provide the tool to track back to the location and nature of the primary JH action.