Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Mar 7;151(2):175-9.
doi: 10.1007/BF00338692.

Rapid screening for plasmid DNA

C Hughes, G G Meynell

Rapid screening for plasmid DNA

C Hughes et al. Mol Gen Genet. .

Abstract

A procedure is described for demonstrating plasmid DNA and its molecular weight, based on rate zonal centrifugation of unlabelled DNA in neutral sucrose gradients containing a low concentration of ethidium bromide. Each DNA species is then visualized as a discrete fluorescent band when the centrifuge tube is illuminated with ultra-violet light. Plasmids exist as closed circular and as relaxed circular molecules, which sediment separately, but during preparation of lysates, closed circular molecules are nicked so that each plasmid forms only a single band of relaxed circles within the gradient.

PubMed Disclaimer

References

    1. Mol Gen Genet. 1973 Sep 12;125(3):217-30 - PubMed
    1. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 - PubMed
    1. J Mol Biol. 1968 Apr 14;33(1):141-71 - PubMed
    1. Biopolymers. 1970;9(3):373-9 - PubMed
    1. Biochim Biophys Acta. 1974 May 17;349(2):145-7 - PubMed

LinkOut - more resources