Monoclonal antibody C38 recognizes retinal ganglion cells in cats and rats

Abstract

We developed monoclonal antibody C38 which specifically recognizes retinal ganglion cells (RGCs) in flatmount preparations of cat and rat retinas. We first induced immunological tolerance in Balb/c mice against axotomized rat retinas which lack most of the RGCs. Then the mice were immunized with intact rat retinas to produce antibodies against RGCs. Monoclonal antibody C38 appeared to be specific for cat RGCs based on immunoreactivities seen in flatmounts and vertical sections of the retina. In rats, we verified that over 90% of retrogradely labeled RGCs were immunoreactive for C38 antibody. In axotomized rat retinas, surviving RGCs were labeled with C38 without erroneous labeling of glial cells. The antigen that C38 recognized was 24 kDa in molecular weight and found in cerebrum, cerebellum, and spinal cord as well as retina. It is suggested that monoclonal antibody C38 is a useful label for RGCs.