Engineering hybrid pseudomonads capable of utilizing a wide range of aromatic hydrocarbons and of efficient degradation of trichloroethylene
- PMID: 8763929
- PMCID: PMC178158
- DOI: 10.1128/jb.178.14.4039-4046.1996
Engineering hybrid pseudomonads capable of utilizing a wide range of aromatic hydrocarbons and of efficient degradation of trichloroethylene
Abstract
We constructed hybrid Pseudomonas strains in which the bphA1 gene (coding for a large subunit of biphenyl dioxygenase) is replaced with the todC1 gene (coding for a large subunit of toluene dioxygenase of Pseudomonas putida Fl) within chromosomal biphenyl-catabolic bph gene clusters. Such hybrid strains gained the novel capability to grow on a wide range of aromatic hydrocarbons, and, more interestingly, they degraded chloroethenes such as trichloroethylene and cis-1,2-dichloroethylene very efficiently.
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