Intracellular calcium stores in isolated tracheal smooth muscle cells

Abstract

We investigated the effects of acetylcholine (ACh) and histamine on intracellular calcium concentration ([Ca2+]i) and contraction of freshly isolated guinea pig tracheal smooth muscle cells. Previous electrophysiological studies revealed that agonists elicit cation and Cl- currents, but a role for Ca2+ in mediating these effects remains unresolved. Here we characterize agonist-induced changes of [Ca2+]i, using fura 2, and examine the contribution of the sarcoplasmic reticulum (SR) to regulation of [Ca2+]i. We provide evidence that the rise of [Ca2+]i and the contraction elicited by ACh or histamine are largely due to release of Ca2+ from stores. Agonists elicited Ca2+ transients in Ca(2+)-free solution with 0.5 mM ethylene glycol-bis (beta-aminoethyl ether)-N, N, N',N'-tetraacetic acid (EGTA), whereas prolonged exposure to Ca(2+)-free solution diminished the rise of [Ca2+]i. In addition, blockade of SR Ca(2+)-adenosinetriphosphatase (ATPase) by cyclopiazonic acid (CPA) or thapsigargin caused elevation of [Ca2+]i and reduction of ACh-evoked increase of [Ca2+]i. In many cells, [Ca2+]i fell below baseline (undershoot) after ACh or caffeine. CPA abolished this undershoot and reduced the rate of recovery of [Ca2+]i to basal levels. Furthermore, oscillations of [Ca2+]i were elicited in the presence or absence of extracellular Ca2+, and these too were reversibly abolished by CPA. Our results provide evidence that Ca2+ stores play a significant role in agonist-mediated increase of [Ca2+]i in tracheal muscle and that the SR contributes to the restoration of basal Ca2+ levels.