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. 1996 Feb;34(2):482-3.
doi: 10.1128/jcm.34.2.482-483.1996.

New PCR assay for rapid and quantitative detection of human cytomegalovirus in cerebrospinal fluid

J U Vogel  1 , J Cinatl, A Lux, B Weber, A J Driesel, H W Doerr
Affiliations

New PCR assay for rapid and quantitative detection of human cytomegalovirus in cerebrospinal fluid

J U Vogel et al. J Clin Microbiol. 1996 Feb.

Abstract

Rapid Chelex extraction combined with an automated hybridization assay for the detection of PCR-amplified human cytomegalovirus DNA from cerebrospinal fluid was established. Quantitation of DNA was performed with a plasmid being used as an external standard. The detection limit was 10 copies per microliter. Quantitative detection of human cytomegalovirus DNA could be achieved over a range from 10 to 10(4) copies per microliter.

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References

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