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. 1996 Jul;6(7):633-8.
doi: 10.1101/gr.6.7.633.

Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content

N Baskaran  1 , R P Kandpal, A K Bhargava, M W Glynn, A Bale, S M Weissman
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Free article

Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content

N Baskaran et al. Genome Res. 1996 Jul.
Free article

Abstract

A PCR method for uniform amplification of a mixture of DNA templates differing in GC content is described using the two enzyme approach (Klentaq1 and Pfu DNA polymerase) and a combination of DMSO and betaine. This method was applied to amplify the CGG repeat region from the fragile X region.

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