Analysis of ochratoxin B alone and in the presence of ochratoxin A, using carboxypeptidase A
- PMID: 879783
- PMCID: PMC170870
- DOI: 10.1128/aem.33.6.1275-1277.1977
Analysis of ochratoxin B alone and in the presence of ochratoxin A, using carboxypeptidase A
Abstract
A method is described for ochratoxin B analysis, which is adapted to the earlier described method of ochratoxin A analysis, using carboxypeptidase A (K. Hult and S. Gatenbeck, J. Assoc. Off. Anal. Chem. 59:128-129, 1976). The fluorescence spectra of ochratoxins A and B coincide too much to allow direct discrimination of the two compounds. A method using the differences in kinetic parameters of the enzymatic hydrolysis of the two compounds is suggested for the analysis of mixtures of the ochratoxins.
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