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. 1996 Jun;74(3):298-300.
doi: 10.1038/icb.1996.52.

Antigen-specific IgA-secreting cells induced by peripheral vaccination

J S Rothel  1 , L A Corner, H F Seow, P R Wood, M W Lightowlers
Affiliations

Antigen-specific IgA-secreting cells induced by peripheral vaccination

J S Rothel et al. Immunol Cell Biol. 1996 Jun.

Abstract

Antigen-specific IgA-secreting cells were shown to be generated in peripheral lymph nodes following s.c. vaccination. The efferent duct of prefemoral lymph nodes of sheep were cannulated prior to vaccination at a site draining to the cannulated node. Vaccines were contained a recombinant protein (Taenia ovis 45W-GST) and either incomplete Freund's, Quil A or Al(OH)3 as adjuvant. Lymph fluid was examined for the presence of 45W-GST-specific antibody by ELISA and antibody-secreting cells by enzyme-linked immunospot (ELISPOT) assay. Large numbers of anti-45W-GST IgA-secreting cells were detected at various times after vaccination as were IgM, IgG1 and IgG2-secreting cells. For sheep vaccinated using incomplete Freund's adjuvant, up to 11% (> 1 million/day) of 45W-GST-specific ELISPOT were IgA-secreting cells.

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