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. 1977 Jun;15(5-6):455-76.
doi: 10.1007/BF00520191.

Expression of alpha-D-mannosidase in man-hamster somatic cell hybrids

P H Ingram, G A Bruns, V M Regina, R E Eisenman, P S Gerald

Expression of alpha-D-mannosidase in man-hamster somatic cell hybrids

P H Ingram et al. Biochem Genet. 1977 Jun.

Abstract

Two types of alpha-D-mannosidase isozymes are present in human white blood cells, human diploid fibroblasts, and HeLa cells. One of these (the S isozyme) constitutes the major alpha-D-mannosidase of the human cells, has a pH optimum of 4.4, and is associated with lysosomes. The other (the F isozyme) is most active at pH 6, is acid labile, and is located in the soluble portion of the cytoplasm. The expression of human lysosomal alpha-D-mannosidase was examined in man-hamster hybrid clones, and was found to be concordant with that of phosphohexose isomerase in 54 of 55 primary clones. A locus specifying human lysosomal alpha-D-mannosidase has therfore been assigned to chromosome 19.

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References

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