doi: 10.1042/bj1630635.
A rapid procedure for selectively isolating the major glycoprotein from purified rat brain myelin
- PMID: 880222
- PMCID: PMC1164745
- DOI: 10.1042/bj1630635
Item in Clipboard
A rapid procedure for selectively isolating the major glycoprotein from purified rat brain myelin
Biochem J.
.
Abstract
Treatment of the chloroform/methanol-insoluble residue of rat brain myelin with lithium 3,5-di-iodosalicylate solubilized the major myelin-associated glycoprotein along with most other proteins and glycoproteins. Equilibration of the extract with phenol resulted in the selective partitioning of the major glycoprotein into the aqueous phase, whereas nearly all of the other proteins and glycoproteins went into the phenol phase.
Similar articles
-
Purification and partial characterization of the myelin-associated glycoprotein from adult rat brain.Biochim Biophys Acta. 1983 May 4;757(1):140-3. doi: 10.1016/0304-4165(83)90162-9. Biochim Biophys Acta. 1983. PMID: 6188493
-
Lectin-binding proteins in central-nervous-system myelin. Binding of glycoproteins in purified myelin to immobilized lectins.Biochem J. 1979 Nov 1;183(2):213-21. doi: 10.1042/bj1830213. Biochem J. 1979. PMID: 534494 Free PMC article.
-
Isolation of an integral membrane glycoprotein by chloroform-methanol extraction and C3-reversed-phase high-performance liquid chromatography.Anal Biochem. 1987 Aug 1;164(2):474-81. doi: 10.1016/0003-2697(87)90521-5. Anal Biochem. 1987. PMID: 2445226
-
A protein fraction enriched in all myelin associated glycoproteins from adult rat central nervous system.J Neurochem. 1977 Aug;29(2):355-7. doi: 10.1111/j.1471-4159.1977.tb09631.x. J Neurochem. 1977. PMID: 886338 No abstract available.
-
Carbohydrate moieties of myelin-associated glycoprotein, major glycoprotein of the peripheral nervous system myelin and other myelin glycoproteins potentially involved in cell adhesion.Dev Neurosci. 1992;14(5-6):342-50. doi: 10.1159/000111681. Dev Neurosci. 1992. PMID: 1284853
Cited by
-
Generation and characterization of mouse monoclonal antibodies to the myelin-associated glycoprotein (MAG).Neurochem Res. 1985 Apr;10(4):499-513. doi: 10.1007/BF00964654. Neurochem Res. 1985. PMID: 2582290
-
Conversion of myelin-associated glycoprotein (MAG) to a smaller derivative by calcium activated neutral protease (CANP)-like enzyme in myelin and inhibition by E-64 analogue.Neurochem Res. 1984 May;9(5):629-35. doi: 10.1007/BF00964509. Neurochem Res. 1984. PMID: 6206410
-
The myelin-associated glycoproteins: membrane disposition, evidence of a novel disulfide linkage between immunoglobulin-like domains, and posttranslational palmitylation.J Cell Biol. 1990 Dec;111(6 Pt 1):2651-61. doi: 10.1083/jcb.111.6.2651. J Cell Biol. 1990. PMID: 1703542 Free PMC article.
-
Monoclonal IgM from patients with peripheral demyelinating neuropathies cross-react with bacterial polypeptides.Clin Exp Immunol. 1994 Jun;96(3):466-9. doi: 10.1111/j.1365-2249.1994.tb06052.x. Clin Exp Immunol. 1994. PMID: 8004816 Free PMC article.
-
Preparation and characterization of antisera to the myelin-associated glycoprotein.Neurochem Res. 1981 Oct;6(10):1115-27. doi: 10.1007/BF00964417. Neurochem Res. 1981. PMID: 6174880
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
