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. 1979 Apr;6(4):1309-21.
doi: 10.1093/nar/6.4.1309.

A new method for the size estimation of the RNA genome segments of influenza virus

M J Sleigh, G W Both, G G Brownlee
Free PMC article

A new method for the size estimation of the RNA genome segments of influenza virus

M J Sleigh et al. Nucleic Acids Res. 1979 Apr.
Free PMC article

Abstract

Previous estimates of the size of the RNA genome segments of influenza virus have been unreliable because of a lack of suitable RNA species as size markers. We have attempted to overcome this problem by utilising the ability of AMV reverse transcriptase to synthesise full length DNA copies of RNA molecules in the presence of a suitable primer. By comparing such DNA copies of the RNA segments of the influenza virus genome with sequenced restriction fragments from the E. coli plasmid pBR322, we have made more reliable estimates of the sizes of the eight genome segments from influenza virus A/NT/60/68.

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References

    1. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3045-9 - PubMed
    1. J Virol. 1976 Mar;17(3):876-84 - PubMed
    1. Virology. 1976 Feb;69(2):789-92 - PubMed
    1. Virology. 1961 Jan;13:53-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5145-9 - PubMed