Spermatogenesis and the role of Sertoli cells in the freshwater snail Biomphalaria glabrata

Abstract

The various stages of spermatogenesis and the Sertoli cells of Biomphalaria glabrata were studied with histochemical and electron microscope techniques. During spermatogenesis a manchette of microtubules is formed around the nucleus and the mid-piece of the spermatids. This manchette becomes helically coiled and probably plays an important role in the spiralisation of the nucleus and of the mitochondrial sheath. During spermatogenesis so-called chromatoid bodies (CB) occur, which consist of arginine-rich proteins. These CB disintegrate during the early spermatid stage. The results suggest that the CB are either involved in histone transition or in the formation of microtubules. The remaining cytoplasm of the spermatids is phagocytised by the Sertoli cells. Apparently this process of phagocytosis is an important part of the mechanism of spermiation. Morphological measurements of the Sertoli cells showed that the relative volume of most organelles decrease during spermatogenesis, indicating a general decrease in cell activity. Possible functions of the Sertoli cells, such as transportation and nutrition of spermatogenic cells and hormone production, are discussed. It is concluded on the basis of the histochemical and ultrastructural observations that the Sertoli cells are involved in the nutrition of spermatogenic cells. It seems unlikely that they are hormone producing cells.