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. 1996 Jun 15;52(1-2):91-103.
doi: 10.1016/0165-2427(95)05535-5.

Identification of gamma delta T lymphocyte subsets that populate calf ileal mucosa after birth

C R Wyatt  1 , E J Brackett, L E Perryman, W C Davis
Affiliations

Identification of gamma delta T lymphocyte subsets that populate calf ileal mucosa after birth

C R Wyatt et al. Vet Immunol Immunopathol. .

Abstract

Ileal intraepithelial and lamina propria lymphocytes from newborn, 1.5-week-old, and 3-week-old calves were compared to determine to what extent the mucosa becomes populated after birth. Single and dual fluorescence flow cytometry were used with monoclonal antibodies to bovine (Bo) CD molecules to identify lymphocyte subpopulations. Few ileal mucosal lymphocytes were present in calves at birth. However, by 1.5 weeks of age, the villi were populated with large numbers of lymphocytes, and by 3 weeks of age, the numbers had increased further. These included a prominent subpopulation of gamma delta T cells. Several subsets of gamma delta T cells populated ileal mucosa after birth. The predominant subset coexpressed BoCD2, and a smaller subset coexpressed BoCD8. WC1+ gamma delta T cells comprised the smallest subset. All gamma delta T cell subsets coexpressed ACT2, a molecule expressed on activated WC1+ and WC1- gamma delta T cells from cattle.

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References

    1. Baldwin C.L., Teale A.J., Naessens J.G., Goddeeris B.M., MacHugh N.D., Morrison W.I. Characterisation of a subset of bovine T lymphocytes that express BoT4 by monoclonal antibodies and function: similarity to lymphocytes defined by human T4 and murine L3T4. J. Immunol. 1986;136:4385–4391. - PubMed
    1. Bandeira A., Itohara S., Bonneville M., Burlen-Defranoux O., Mota-Santos T., Coutinho A., Tonegawa S. Vol. 88. 1991. Extrathymic origin of intestinal intraepithelial lymphocytes bearing T-cell antigen receptor γδ; pp. 43–47. (Proc. Natl. Acad. Sci. USA). - PMC - PubMed
    1. Bjorneby J.M., Riggs M.W., Perryman L.E. Cryptosporidium parvum merozoites share neutralization-sensitive epitopes with sporozoites. J. Immunol. 1990;145:298–304. - PubMed
    1. Bland P.W., Warren L.G. Antigen presentation by epithelial cells of the rat small intestine. II. selective induction of suppressor T cells. Immunology. 1986;58:9–14. - PMC - PubMed
    1. Chiodini R.J., Davis W.C. The cellular immunology of bovine paratuberculosis: the predominant response is mediated by cytotoxic gamma/delta T lymphocytes which prevent CD4+ activity. Microb. Pathogenesis. 1992;13:447–463. - PubMed

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