Ultrastructural characterization of pharyngeal and esophageal motoneurons in the nucleus ambiguus of the rat

Abstract

The viscerotopic organization of the upper alimentary tract has been established in the nucleus ambiguus, but there is little information about the morphology of the individual neurons innervating the pharynx and esophagus. We studied the ultrastructure of pharyngeal (PH), cervical esophageal (CE), and subdiaphragmatic esophageal (SDE) motoneurons labeled by retrogradely transported wheat germ agglutinin conjugated horseradish peroxidase (WGA-HRP) in the compact formation of the nucleus ambiguus. WGA-HRP was injected into the lower pharynx, or the cervical and subdiaphragmatic esophagus of male rats. The retrogradely labeled PH neurons in the rostral portion of the compact formation were large (26.1 x 50.1 microns, 906.7 microns2), polygonal, and contained well-developed cell organelles with a round nucleus. Subsurface cisterns connected with rough endoplastic reticulum were often present near the postsynaptic membrane. Both CE and SDE neurons in the compact formation were medium-sized, round or oval, and contained well-developed cell organelles, although the SDE neuron was significantly larger than the CE neuron (24.9 x 33.6 microns, 593.0 microns2 in the SDE neuron, and 19.5 x 30.2 microns, 440.3 microns2 in the CE neuron). The average number of axosomatic terminals in a sectional plane was largest in PH neurons (29.0), smaller in CE neurons (7.9), and smallest in SDE neurons (4.2). The number of axosomatic terminals containing round vesicles (Gray's type I) was almost equal to that of terminals containing pleomorphic vesicles (Gray's type II) in PH and CE neurons, but there were few Gray's type II axosomatic terminals in SDE neurons. Desmosome-like junctions at somato-somatic or somato-dendritic apposition were often present in the area surrounding SDE neurons. There were also small unlabeled neurons (9.5 x 18.1 microns, 131.8 microns2) in the compact formation of the nucleus ambiguus. The small neurons contained poorly developed cell organelles and an irregular shaped nucleus with invaginated nuclear membrane, and had no Nissl bodies. These results indicate that PH neurons have the characteristics of somatic motoneurons, and that CE and SDE neurons are similar to visceral motoneurons.