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Comparative Study
. 1996 Apr;34(4):866-9.
doi: 10.1128/jcm.34.4.866-869.1996.

Rapid identification of mycobacterial species by PCR amplification of hypervariable 16S rRNA gene promoter region

P Dobner  1 , K Feldmann, M Rifai, T Löscher, H Rinder
Affiliations
Comparative Study

Rapid identification of mycobacterial species by PCR amplification of hypervariable 16S rRNA gene promoter region

P Dobner et al. J Clin Microbiol. 1996 Apr.

Abstract

A total of 0.3 to 0.4 kb of the promoter region of the 16S rRNA genes from Mycobacterium tuberculosis, M. gordonae, M. xenopi, and M. leprae was PCR amplified, cloned, and sequenced. The observed number of substitutions, insertions, and deletions exceeded those found in previously used target sequences, including the entire 16S coding region. A simple and generally applicable restriction fragment length polymorphism method that can be used to distinguish between mycobacterial species is described.

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