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. 1996 Apr;34(4):980-2.
doi: 10.1128/jcm.34.4.980-982.1996.

Host DNA can interfere with detection of Borrelia burgdorferi in skin biopsy specimens by PCR

F B Cogswell  1 , C E Bantar, T G Hughes, Y Gu, M T Philipp
Affiliations

Host DNA can interfere with detection of Borrelia burgdorferi in skin biopsy specimens by PCR

F B Cogswell et al. J Clin Microbiol. 1996 Apr.

Abstract

It is demonstrated that a diagnostic PCR for Borrelia burgdorferi can be inhibited in the presence of more than 500 ng of host (monkey skin) DNA. The inhibitor is the host DNA itself. An acceptable value for analytical sensitivity can be obtained by diluting the skin-B. burgdorferi proteinase K lysate to a level below the inhibitory concentration of the host DNA. Dilution of the lysate may obviate the need for further DNA purification.

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References

    1. J Clin Microbiol. 1994 Mar;32(3):715-20 - PubMed
    1. J Invest Dermatol. 1995 Jan;104(1):23-6 - PubMed
    1. Lab Invest. 1995 Feb;72(2):146-60 - PubMed
    1. J Clin Microbiol. 1994 Dec;32(12):2980-8 - PubMed
    1. Eur J Clin Microbiol Infect Dis. 1995 Jan;14(1):1-5 - PubMed

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