Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1996 Sep 24;226(3):638-44.
doi: 10.1006/bbrc.1996.1408.

DNA bending between upstream activator sequences increases transcriptional synergy

Affiliations

DNA bending between upstream activator sequences increases transcriptional synergy

J Kim et al. Biochem Biophys Res Commun. .

Abstract

To determine if DNA bending enhances transcriptional synergy between tandem upstream activators, we constructed a series of synthetic promoters containing either an AP1 or an NF1 site linked to four copies of the putative transcription factor binding site ACGTGA incorporated into an intrinsic DNA bending sequence. In transient transfection, the intrinsic bending sequence between AP1 and the TATA box or between NF1 and the TATA box elicited a strong synergistic activation of transcription. The synergistic activation of transcription was greatly reduced when the intrinsic bending sequence was replaced with a mutant sequence which retains the binding site, but does not bend the DNA, and when the intrinsic bending sequence was upstream of the AP1 or NF1 sites. These data indicate that bent DNA located between upstream activator sequences can facilitate transcriptional synergism between bound activators.

PubMed Disclaimer

Publication types

MeSH terms

Substances

LinkOut - more resources