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Comparative Study
. 1996 Jul;12(9):893-8.
doi: 10.1002/(SICI)1097-0061(199607)12:9%3C893::AID-YEA973%3E3.0.CO;2-I.

A Candida albicans gene encoding a DNA ligase

E Andaluz  1 , G Larriba, R Calderone
Affiliations
Comparative Study

A Candida albicans gene encoding a DNA ligase

E Andaluz et al. Yeast. 1996 Jul.

Abstract

A DNA ligase-encoding gene (Ca CDC9) was cloned from Candida albicans by complementation of an ime-1 mutation in Saccharomyces cerevisiae. In this system, IME1 function was assayed using a S. cerevisiae strain with a ime2-promoter-lacZ gene fusion such that following transformation with a C. albicans genomic library, the presence of positive clones was indicated upon the addition of X-gal to sporulation media. Transforming fragments were subcloned in pGEM7 and sequenced. Sequence homology with several ATP-dependent DNA ligases from viruses, fission yeast, human, baker yeast and bacteria was observed.

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