The centrosome in animal cells and its functional homologs in plant and yeast cells

Abstract

The centrosome is the principal microtubule-organizing center in mammalian cells. Until recently, the centrosome could only be studied at the ultrastructural level and defined as a functional entity. However, during the past decade a number of clever experimental strategies have been used to identify numerous molecular components of the centrosome. The identification of biochemical subunits of the centrosome complex has allowed the centrosome to be investigated in much more detail, resulting in important advances being made in our understanding of microtubule nucleation events, spindle formation, the assembly and replication of the centrosome, and the nature of the microtubule-organizing centers in plant cells and lower eukaryotes. The next several years should see additional rapid progress in our understanding of the microtubule cytoskeleton as investigators begin to assign functions to the centrosome proteins that have already been reported and as additional centrosome components are discovered.