[Thin-layer chromatography of sugars (author's transl)]

Abstract

A thin-layer chromatographic method for the detection of sugars is described. The method can also be adapted for the use in biochemistry, pharmacology, and food chemistry. The solvent systems are: I, benzene/1-butanol/acetone/water (20 : 30 : 40 : 10, v/v); II, 1,2-dichloroethane/glacial acetic acid/methanol/water (50 : 25 : 15 : 10, v/v); III, boric acid 0.5%/1-butanol/2-propanol (20 : 30 : 50, v/v). Detection is developed by a diphenylamine aniline phosphoric acid reagent. Additionally an enzymatic method for the detection of some sugars is described.