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. 1977 Jul 15;78(2):203-7.
doi: 10.1016/0009-8981(77)90307-2.

The simultaneous quantitative determination of cephalothin and cefazolin in serum by high pressure liquid chromatography

J S Wold, S A Turnipseed

The simultaneous quantitative determination of cephalothin and cefazolin in serum by high pressure liquid chromatography

J S Wold et al. Clin Chim Acta. .

Abstract

Conventional microbiological assay procedures for cephalosporins in serum do not allow the determination of serum concentrations if more than one cephalosporin is present in a single sample. An HPLC procedure has been developed which permits the simultaneous quantitative determination of cefazolin sodium and cephalothin sodium in serum. Reverse phase chromatography using methanol in 0.2 M ammonium acetate as the mobile phase was employed to separate and quantitate the two cephalosporins in a trichloroacetic acid supernatant solution prepared from serum.

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