Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Aug 1;78(3):479-87.
doi: 10.1016/0009-8981(77)90081-x.

Sulfhaemoglobin. Absorption spectrum, millimolar extinction coefficient at lambda = 620 nm, and interference with the determination of haemoglobin and of haemiglobincy anide

P Dijkhuizen, A Buursma, A M Gerding, W G Zijlstra

Sulfhaemoglobin. Absorption spectrum, millimolar extinction coefficient at lambda = 620 nm, and interference with the determination of haemoglobin and of haemiglobincy anide

P Dijkhuizen et al. Clin Chim Acta. .

Abstract

The spectrophotometry properties of sulfhaemoglobin (SHb) and some derivatives were investigated using an improved technique for measuring the SHb fraction induced in human blood samples. The millimolar extinction coefficient of SHb at lambda = 620 nm was found to be 20.8 (S.D. 1.48; S.E. = 0.44; n = 11). In addition it was demonstrated that the spectral changes occurring in SHb containing haemoglobin solutions upon the addition of KCN, K3Fe(CN)6 and K3Fe(CN)6 +KCN invalidate the KCN addition method for the determination of haemiglobin. The influence of clinically occurring SHb fractions on the internationally standarized total haemoglobin determination were shown to be insignificant.

PubMed Disclaimer

LinkOut - more resources