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. 1977 Jul 8;62(3):243-53.
doi: 10.1007/BF00286046.

Biochemical analysis of meiosis in the male mouse. I. Separation of DNA labelling of specific spermatogenic stages

A C Chandley, Y Hotta, H Stern

Biochemical analysis of meiosis in the male mouse. I. Separation of DNA labelling of specific spermatogenic stages

A C Chandley et al. Chromosoma. .

Abstract

Spermatogenic cells of the mouse have been separated by gravity sedimentation using a modification of a previously published method. Details are given for the collection of purified samples of specific meiotic stages and for the collection of labelled cell fractions following injection of 3H-thymidine. Suppression of semi-conservative meiotic DNA synthesis, essential to the biochemical analysis of pachytene DNA metabolism, has been achieved by in vivo administration of 1 M hydroxyurea.

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References

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